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. 2017 Nov 10;8:258. doi: 10.1186/s13287-017-0693-0

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Transwell assay for hBMSC migration. a Analysis for the migration of hBMSCs with or without hUVECs and hUASMCs. b In the coculture system of hBMSCs and hUASMCs, representative light photomicrographs of migrated hBMSC induced by 0-100 ng/ml TGFβ3 after 24-hour incubation. c In the coculture system of hBMSCs and hUVECs, representative light photomicrographs of migrated hBMSCs induced by 0-100 ng/ml TGFβ3 after 24-hour incubation. d Quantitative analysis of migrated cell density for (c) and (d). Migrated cells were stained purple with crystal violet. Scale bar: 100 μm. *P < 0.05, **P < 0.01, ***P < 0.005, ***P < 0.001. hUASMC human umbilical artery smooth muscle cell, hUVEC human umbilical vein endothelial cell, MSC mesenchymal stem cell, TGFβ3 transforming growth factor beta-3