Fig. 4.

ETV1 is a direct target of miR-17-5p in TNBC cells. a, Target sequences of miR-17-5p in ETV1 3′-UTR and mutant sites in 3′-UTR. b, Relative luciferase activity of ETV1 3′-UTR and mutant in the miR-17-5p mimic-transfected 293 T cells. c, d, The effect of miR-17-5p on ETV1 expression in MDA-MB-231 and BT549 cells was detected by qRT-PCR and western blotting after the cells were transfected with miR-17-5p mimic or inhibitor, respectively. e, The effect of miR-17-5p on ETV1 expression was also observed in MCF10A cells by co-transfecting with GV141-ETV1 and miR-17-5p inhibitor. Data are expressed as the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01