Fig. 7.

miR-17-5p suppressed TNBC cells metastasis in vivo by targeting ETV1 and the potential pathway. a, Control oligos (50 nmol/L) or miR-17-5p mimic (50 nmol/L) transfected MDA-MB-231 cells were labelled with fluorescent dye CM-Dil and then microinjected into the perivitelline spaces of 48-hpf zebrafish embryos. The zebrafish embryos were anaesthetized and imaged using microscopy at 48 h after injection. b, miR-17-5p transcription might be activated by c-Myc, and repressed by p53. Up-regulation of miR-17-5p could inhibit ETV1expression, and negative regulate c-Myc transcription. Finally, miR-17-5p could suppress TNBC development by targeting ETV1 through decreasing MMP, COX-2 and VEGF expression. ** P < 0.01, compared with the control