Table 2.
Fold change expression of microRNAs
| miRNA | RA vs HC | FDR vs HC | FDR vs RA | ||||
|---|---|---|---|---|---|---|---|
| P Value | Fold change | Rank | P Value | Fold change | P Value | Fold change | |
| hsa-miR-103a-3p | 0.0064 | 3.96 | 1 | 0.0238 | 7.68 | 0.1223 | 1.97 |
| hsa-miR-155 | 0.0002 | 2.47 | 2 | 0.0115 | 1.98 | 0.3627 | −1.25 |
| hsa-miR-29b | 0.0648 | 1.91 | 3 | 0.8636 | −1.55 | 0.0754 | −2.96 |
| hsa-miR-132 | 0.0016 | 1.90 | 4 | 0.0687 | 1.37 | 0.2530 | −1.39 |
| hsa-miR-26b-3p | 0.0010 | 1.88 | 5 | 0.0024 | 2.28 | 0.2530 | 1.21 |
| hsa-miR-152 | 0.0038 | 1.83 | 6 | 0.1309 | 1.97 | 0.4981 | 1.08 |
| hsa-miR-19a | 0.0732 | 1.73 | 7 | 0.0205 | 1.55 | 0.9662 | −1.11 |
| hsa-Let-7a | 0.0569 | 1.73 | 7 | 0.2086 | 1.17 | 0.2358 | −1.47 |
| hsa-miR-19b | 0.0260 | 1.67 | 9 | 0.1169 | 1.34 | 0.4091 | −1.24 |
| hsa-miR-146a-5p | 0.0083 | 1.54 | 10 | 0.0031 | 1.99 | 0.3408 | 1.29 |
| hsa-miR-451 | 0.0076 | 1.53 | 11 | 0.0127 | 1.56 | 0.7031 | 1.02 |
| RNU44 | 0.0120 | 1.52 | 12 | 0.0162 | 1.30 | 0.9157 | −1.17 |
| hsa-miR-125a-5p | 0.0272 | 1.30 | 13 | 0.0553 | 1.50 | 0.7508 | 1.15 |
| hsa-miR-222 | 0.0796 | 1.29 | 14 | 0.2154 | 1.20 | 0.4587 | −1.07 |
| hsa-miR-107 | 0.0576 | 1.28 | 15 | 0.2481 | −1.02 | 0.4587 | −1.31 |
| hsa-miR-29c | 0.2428 | 1.28 | 16 | 0.1457 | 1.11 | 0.8159 | −1.15 |
| hsa-Let-7e | 0.0502 | 1.27 | 17 | 0.5837 | −1.20 | 0.0987 | −1.53 |
| hsa-miR-21 | 0.0261 | 1.24 | 18 | 0.0924 | 1.22 | 0.7832 | −1.16 |
| hsa-miR-223 | 0.0115 | 1.22 | 19 | 0.5487 | 1.03 | 0.5115 | −1.82 |
| hsa-miR-26b-5p | 0.1191 | 1.21 | 20 | 0.6744 | −1.45 | 0.0277 | −1.76 |
| hsa-miR-323-3p | 0.1123 | 1.17 | 21 | 0.2033 | 1.12 | 0.9831 | −1.04 |
| hsa-miR-26a | 0.1849 | 1.14 | 22 | 0.1159 | 1.33 | 0.8822 | 1.16 |
| hsa-miR-29a | 0.1031 | 1.14 | 23 | 0.6174 | −1.29 | 0.1124 | 1.46 |
| hsa-miR-15a | 0.5594 | 1.11 | 24 | 0.8292 | −1.56 | 0.1223 | −1.72 |
| hsa-miR-150 | 0.6104 | 1.10 | 25 | 0.41 | 1.20 | 0.7669 | 1.09 |
| hsa-miR-34a* | 0.1065 | 1.06 | 26 | 0.0495 | −1.84 | 0.0262 | −1.94 |
| hsa-miR-221 | 0.1842 | 1.06 | 27 | 0.9914 | −1.37 | 0.2356 | −1.45 |
| hsa-miR-24 | 0.2956 | −1.05 | 28 | 0.8651 | 1.03 | 0.9831 | 1.04 |
| hsa-miR-18a | 0.7615 | −1.07 | 29 | 0.7639 | −1.85 | 0.1124 | −1.72 |
| U6 | 0.8069 | −1.11 | 30 | 0.0001 | −1.59 | 0.0625 | −1.43 |
| hsa-miR-125a-3p | 0.1654 | −1.20 | 31 | 0.6178 | −1.10 | 0.4847 | 1.10 |
| hsa-miR-16 | 0.9950 | −3.94 | 32 | 0.8276 | −1.24 | 0.2802 | −1.23 |
| hsa-miR-346 | 0.0001 | −8.70 | 33 | 0.0001 | −20.00 | 0.0338 | −2.32 |
Abbreviations: FDR First-degree relative, HC Healthy control subjects, miRNA or miR MicroRNA, RA Rheumatoid arthritis
miRNA profiling was analyzed in the whole blood total RNA samples obtained from HC, patients with RA, and FDRs using TaqMan probes. P values were obtained using the Mann-Whitney U test, and median fold change values were used to classify miRNAs rankwise. Columns highlighted in bold represent miRNAs that show significant differential expression after Benjamini-Hochberg correction for multiple comparison analyses (Q < 0.04). RA vs HC indicates miRNA expression in patients with RA compared with HCs. FDR vs HC indicates miRNA expression in FDRs compared with HCs. FDR vs RA indicates miRNA expression in FDRs compared with patients with RA. hsa-miR-34a* represents hsa-miR-34a-3p