Figure 7.

EA inhibited NF-κB signaling pathway by upregulating CYLD. (A) Western blot of CYLD, p-IκBα, IκBα, nuclear and cytoplasmic NF-κB p65 expression after 24 h reperfusion. Total and cytoplasm proteins normalized with β-actin and nuclear protein normalized with lamin B. (B) CYLD expression, ratio of p-IκBα and nuclear translocation ratios of NF-κB p65. *p < 0.001, ^#p < 0.05, ^$p < 0.001 vs. MCAO/R + EA group, n = 5/group. (C) Neuronal CYLD and nuclear NF-κB p65 positive cells counts are presented as number/mm². **p < 0.001, ^##p < 0.05 vs. MCAO/R group, ^@@p < 0.05, ^$$p < 0.05 vs. MCAO/R+EA group, n = 5/group. (D) Immunofluorescent co-expression of CYLD (green) and NF-κB p65 (red) after 24 h reperfusion in borders of ischemic areas (Scale bar = 50 μm). White arrows indicated magnified components from merged image, n = 5/group.