Fig. 1.

a, b RNA expression of Ara-C metabolizing genes DCK and CDA were determined using ΔΔCT method, where ΔCT is the difference in the threshold cycle (CT) values of target gene and the housekeeping gene GAPDH. Each sample was normalized to ΔCT value of the sample AML001. Index case is the patient documented with cerebellar toxicity. Median DCK expression was 214.4 (31.87–1113), whereas the index case DCK expression was determined to be 1030. Median CDA expression was 95.92 (0.238–2760), and the index case CDA expression was determined to be 6.56. Taqman-based gene expression assays used were Hs01040725_m1 (DCK), Hs00156401_m1 (CDA), and 4352934E for GAPDH