Skip to main content
. Author manuscript; available in PMC: 2017 Nov 11.
Published in final edited form as: Anal Chem. 2017 May 3;89(10):5484–5493. doi: 10.1021/acs.analchem.7b00423

Figure 1.

Figure 1

Stoichiometric equation for the HRP-catalyzed oxidation of p-phenylenediamine (PPD) with H2O2 to Bandrowski’s base at pH 7.0 in its neutral (BB) and monoprotonated (BBH+) forms. The reported pKa value of BBH+ is 7.4.20 At pH 7.0, the calculated molar ratio of BBH+ to BB is 2.5:1.