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. Author manuscript; available in PMC: 2017 Nov 11.
Published in final edited form as: Anal Chem. 2017 May 3;89(10):5484–5493. doi: 10.1021/acs.analchem.7b00423

Figure 2.

Figure 2

Time-dependent changes in the absorption spectrum of a reaction solution which is kept at 25 °C containing initially 1.5 mM PPD, 20 pM HRP, and 80 μM H2O2 at pH 7.0 (buffer-1). The reaction solution was kept in a 1 cm quartz cell and the spectrum was recorded every min after starting the reaction by adding H2O2 for a total of 10 min. The dashed line indicates λiso; λmax (BB) = 460 nm; λmax (BBH+) = 530−540 nm, see text for details. With ε500 = 1.109 × 104 M−1 cm−1 (see text), A500 (l = 1 cm) = 0.01 corresponds to 0.9 μM Bandrowski’s base.