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HRP activity measurements with p-phenylenediamine (PPD) at 25 °C and pH = 7.0 (buffer-2), with 0.2 mM H2O2 and 0.5 nM HRP. The initial rate of product formation (Bandrowski’s base) is plotted as a function of PPD concentration. Each data point represents the mean and standard deviation from three measurements. The data points were fit with the Michaelis−Menten equation, r2 = 0.9961.
