Figure 4. IL-22 treatment further increases AMP production via STAT3 signaling.
Real-time PCR analysis of anti-microbial peptide gene expression following injury and IL-22 treatment. RNA from ileum epithelial cells was analyzed for expression of (A, E) Reg3β, (B, F) Reg3γ, (C,G) lipocalin-2 transcripts and (D,H) lipocalin protein in wild-type mice, (n=4-10) (A-D) and VillinCre STAT3-/- knockout mice, sham vehicle (n =3-8) (E-H). *p < 0.05, **p < 0.01, ***p < 0.001, by One-Way ANOVA with Tukey post-hoc compared to untreated sham vehicle.