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. 2017 Nov 7;8:2161. doi: 10.3389/fmicb.2017.02161

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Copyright © 2017 Kumari, Mishra, Arora, Chatrath, Gangwar, Roy and Prasad.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal laser scanning microscopic images of C. neoformans and C. laurentii biofilms formed before and after treatment with EO-ACs. (A,E) Control (1% DMSO) (B–D) 32 μg/ml of citral, carvacrol and thymol (F–H) 16 μg/ml of citral, carvacrol and thymol. In the control panels, images of biofilm showed metabolically active (red, FUN-1-stained) cells embedded in the EPM (green, CAAF- 488 -stained). In the citral, carvacrol and thymol treatment panels, the yellow-brownish region represents metabolically non-viable cells. The images were taken by using 40X power field. Scale bar 50 μm.