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. 2017 Nov 7;8:2161. doi: 10.3389/fmicb.2017.02161

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Copyright © 2017 Kumari, Mishra, Arora, Chatrath, Gangwar, Roy and Prasad.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytotoxic effects of EO-ACs on HEK-293. (A) The cytotoxic effect of thymol, carvacrol, and citral on normal human renal cells viability (B) Brightfield and fluorescence microscopy of HEK-293 cells before and after treatment with thymol, carvacrol, and citral at their respective MIC80. Control and treatment panels show fluorescence images stained with acridine orange (AO) and ethidium bromide (EB) for live/dead cells (Green: live cells and Red: dead cells). Yellow arrows indicate dead renal cells. The brightfield images were taken using 10X objective lens and fluorescence images were taken using a 40X objective lens. Scale bar 50 μm.