FIGURE 1.

TBSV-GFP (TG) and TRBO-GFP (TRBO-G) viral vectors. Only the relevant part of the insert in the binary vectors is illustrated. In vivo transcription of the viral (+) sense RNA genomes is driven by the upstream CaMV 35S (35S) promoter; the dark box labeled ‘R’ at the 3′ end represents a ribozyme, ‘An’ refers to the poly (A) signal. The numerical “P” notations indicate the size in kDa of the proteins encoded by the viral genes. Rep Proteins indicate replication-associated proteins; MP, cell-to-cell movement protein; P19, suppressor of RNA silencing. GFP expressed from TG contains 17 extra amino acids that correspond to the remnant N-terminal end of the capsid protein. The asterisks indicate the presence of an amber read-through stop-codon. Further details on TG and TRBO-G were published previously (Lindbo, 2007; Shamekova et al., 2014).