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. 2017 Nov 7;8:1808. doi: 10.3389/fpls.2017.01808

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Copyright © 2017 Mendoza, Payne, Castillo, Crocker, Shaw and Scholthof.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot for detection of GFP in tomato, cowpea and lettuce. TG samples were collected at 3 dpi while Mock, 00 (control Agrobacterium expressing an empty TRV vector), TRBO-G and TG+TRBO-G were collected at 7 dpi. Primary GFP antibodies were used (α-GFP), and Coomassie Brilliant Blue (CB) staining was performed to provide loading comparisons. The red colored size marker (S) on the left of western blots is 25 kDa, while the size of the intense CB band of a host protein is about 55 kDa as inferred from the size marker on the left.