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. 2017 Nov 7;8:904. doi: 10.3389/fphys.2017.00904

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Copyright © 2017 Shi, Qian, Liu, Sun, Wang, Liu, Xu and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunofluorescent analysis of nerve fiber density at 2 weeks post-surgery. (A) Representative immunofluorescent images of neurofilament heavy chain (red fluorescence) were detected at 2 weeks post-surgery. Nuclei were stained with DAPI (blue fluorescence). The 200× image is the magnified view of the area denoted by the dashed boxes in the 100× image. Scale bar: 50 μm. (B) Quantitative analysis of the nerve fiber density in each group at 2 weeks post-surgery. a, P < 0.05 compared to the control group; b, P < 0.05 compared to the hydrogel group.