FIGURE 6.

Comparative mapping of Pm21. (A) Genetic map (partial) using the F₂ population derived from the cross between the resistant D. villosum line DvRes-1 and the susceptible line DvSus-1. The dark region on the chromosome 6VS demonstrates the genetic interval carrying Pm21. The vertical arrow shows the direction of the centromere of 6V. The complete genetic map can be found in Figure 3A. (B–D) Comparative maps of the Pm21 locus among D. villosum 6VS, wheat 6AS, 6BS, and 6DS (B), the short arms of Brachypodium chromosome 3 (C) and rice chromosome 2 (D). The homologous wheat contigs of genes of interest were obtained and annotated by using the BLAST, FGENESH, and SMART programs (Table 2). All genes from Brachypodium and rice are adopted according to the annotations of the corresponding genomes except the RGA Bradi3g03935 in Brachypodium that was re-annotated in this study. The solid circles and hollow circles indicate conserved genes and unconserved genes among different genomes, respectively. The orthologous RGAs among D. villosum, wheat and Brachypodium are marked by red stars.