Table 2.
Statistical analysis of enzyme activity in children with Autism Spectrum Disorders (ASD). On average, Citrate synthase was significantly higher and Complex I and IV activity normalized to citrate synthase activity were significantly lower for ASD participants as compared to controls. The variability in enzyme activity was significantly higher for all enzymes in ASD participants as compared to controls. Also significantly more enzyme activity values were outside the normal range for ASD participants as compared to the control range
| Citrate synthase (nmol/min/mg protein | Complex I activity (% citrate synthase activity) | Complex IV activity (% citrate synthase activity) | Complex I/IV ratio | Any abnormal | |
|---|---|---|---|---|---|
| Control Mean (SD) | 12.9 (5.1) | 6.7 (2.0) | 0.33 (0.10) | 21.2 (9.1) | |
| ASD Mean (SD) | 16.5 (9.0) | 5.4 (3.5) | 0.27 (0.19) | 25.3 (21.4) | |
| Mean Difference (t142, p) | 3.04, <0.005 | 2.80, <0.01 | 2.26, <0.05 | 1.51, =0.13 | |
| Variance Difference (F75,67,p) | 3.14, <0.0001 | 3.12,<0.0001 | 3.52, <0.0001 | 5.53,<0.0001 | |
| Normal Range | 2.9–22.9 | 2.8–10.6 | 0.13–0.53 | 3.4–39 | |
| % Above Normal, p value | 22% (17/76) < 0.0001 | 11% (8/76) < 0.0001 | 8% (6/76) < 0.005 | 7% (5/76) < 0.01 | 41% (31/76) < 0.0001 |
| % Below Normal, p value | 3% (2/76), 0.57 | 28% (21/76) < 0.0001 | 3% (2/76) 0.56 | 17% (13/76) < 0.0001 | 45% (34/76) < 0.0001 |
| % Outside Normal, p value | 24% (19/76) < 0.0001 | 39% (29/76) < 0.0001 |
11% (8/76)
=0.01 |
24% (18/76) < 0.0001 | 62% (47/76) < 0.0001 |
Statistically significant values are represented in bold