Table 2.

Secretome of strains THCO2-90 and gldD::Tn identified by LC-MS/MS analysis of cell-free supernatant^a.

Locus tag	Gene	Predicted functionb	CTDc	Ratio TH/gldDd	Spectrum count averagese
					TH	gldD::Tn
THC0290_0237	fpp1	Psychrophilic metalloprotease Fpp1 precursor	TIGR04183	Absent	17.33	0.00
THC0290_1494		Probable ribonuclease	TIGR04183	Absent	13.67	0.00
THC0290_1520		Probable endonuclease precursor	TIGR04183	Absent	10.67	0.00
THC0290_1527		Protein of unknown function precursor containing a C-terminal secretion signal. Putative adhesin	TIGR04131	Absent	10.33	0.00
THC0290_2201		Protein of unknown function precursor, putative adhesin		Absent	10.33	0.00
THC0290_1797		Protein of unknown function precursor	TIGR04183	Absent	9.00	0.00
THC0290_1998		Protein of unknown function precursor		Absent	7.67	0.00
THC0290_0944		Probable S8 subtilisin family serine endopeptidase precursor	TIGR04183	Absent	7.00	0.00
THC0290_1048		Protein of unknown function precursor, putative adhesin	TIGR04131	Absent	5.67	0.00
THC0290_0737		Protein of unknown function		Absent	4.00	0.00
THC0290_0121		Probable lipoprotein precursor		Absent	3.67	0.00
THC0290_0908		Protein of unknown function		Absent	3.67	0.00
THC0290_1054		Protein of unknown function precursor containing a C-terminal secretion signal	TIGR04183	Absent	3.00	0.00
THC0290_0171		Probable cell surface protein (Leucine-rich repeat protein) precursor	TIGR04183	Absent	8.33	0.00
THC0290_2158		Uncharacterized protein precursor. Probable phage protein.		54.00	18.00	0.33
THC0290_0500		Probable S8 and S53 subtilisin family serine endopeptidase precursor		52.67	52.67	1.00
THC0290_0743	gldN	Gliding motility protein precursor GldN		46.50	62.00	1.33
THC0290_0740	gldK	Gliding motility lipoprotein precursor GldK		43.50	58.00	1.33
THC0290_0339	porT	PorT protein		28.00	9.33	0.33
THC0290_0174		Probable cell surface protein (Leucine-rich repeat protein) precursor	TIGR04183	24.00	8.00	0.33
THC0290_2147	gldJ	Gliding motility lipoprotein precursor GldJ		23.67	94.67	4.00
THC0290_0754		Protein of unknown function precursor, putative adhesin		18.25	24.33	1.33
THC0290_1615		Protein of unknown function		18.00	24.00	1.33
THC0290_0291		Protein of unknown function precursor		18.00	6.00	0.33
THC0290_2029		Protein of unknown function precursor		17.50	11.67	0.67
THC0290_0129		Putative outer membrane protein precursor	TIGR04183	17.00	22.67	1.33
THC0290_0091		Probable glycoside hydrolase precursor	TIGR04183	14.00	9.33	0.67
THC0290_2146	porU	Por secretion system protein PorU precursor. C-terminal signal peptidase		12.25	16.33	1.33
THC0290_0931		Collagenase precursor	TIGR04183	11.29	26.33	2.33
THC0290_1526		Protein of unknown function precursor		10.00	3.33	0.33
THC0290_1595		Protein of unknown function precursor, putative adhesin	TIGR04183	9.75	13.00	1.33
THC0290_1616		Protein of unknown function precursor, putative adhesin	TIGR04131	8.33	16.67	2.00
THC0290_0175		Probable cell surface protein (Leucine-rich repeat protein)	TIGR04183	7.75	10.33	1.33
THC0290_0299		Probable M36 fungalysin family metalloprotease precursor	TIGR04183	6.93	106.33	15.33
THC0290_1047		Protein of unknown function precursor, putative adhesin	TIGR04131	6.50	4.33	0.67
THC0290_0176		Probable cell surface protein (Leucine-rich repeat protein) precursor	TIGR04183	6.40	10.67	1.67
THC0290_0238	fpp2	Psychrophilic metalloprotease Fpp2 precursor	TIGR04183	6.15	67.67	11.00
THC0290_0186		Probable cell surface protein (Leucine-rich repeat protein) precursor	TIGR04183	5.40	9.00	1.67
THC0290_0300		Probable M36 fungalysin family metalloprotease precursor	TIGR04183	5.00	6.67	1.33
THC0290_0173		Probable cell surface protein	TIGR04131	4.77	47.67	10.00

^aProteins which relative abundance changed at least ±2-fold significantly (p < 0.01) and for which at least 3 spectra have been identified in one of analyzed strain (full data available in Table S1).

^bProteins annotation as previously described (Rochat et al., 2017a).

^cCTD type identified by BLASTP analysis.

^dProtein abundance ratio calculated using spectral counts.

^eAverage number of spectral counts calculated using triplicates values for each strain.