Table 3.
Secretome of strains THCO2-90 and gldG::Tn identified by LC-MS/MS analysis of cell-free supernatanta.
| Spectrum count averagese | ||||||
|---|---|---|---|---|---|---|
| Locus tag | Gene | Predicted functionb | CTDc | Ratio TH/gldGd | TH | gldG::Tn |
| THC0290_0237 | fpp1 | Psychrophilic metalloprotease Fpp1 precursor | TIGR04183 | Absent | 17.33 | 0.00 |
| THC0290_0944 | Probable S8 subtilisin family serine endopeptidase precursor | TIGR04183 | Absent | 7.00 | 0.00 | |
| THC0290_1048 | Protein of unknown function precursor, putative adhesin | TIGR04131 | Absent | 5.67 | 0.00 | |
| THC0290_0171 | Probable cell surface protein (Leucine-rich repeat protein) precursor | TIGR04183 | Absent | 8.33 | 0.00 | |
| THC0290_2147 | gldJ | Gliding motility lipoprotein precursor GldJ | 40.57 | 94.67 | 2.33 | |
| THC0290_1520 | Probable endonuclease precursor | TIGR04183 | 32.00 | 10.67 | 0.33 | |
| THC0290_0174 | Probable cell surface protein (Leucine-rich repeat protein) precursor | TIGR04183 | 24.00 | 8.00 | 0.33 | |
| THC0290_0740 | gldK | Gliding motility lipoprotein precursor GldK | 17.40 | 58.00 | 3.33 | |
| THC0290_2201 | Protein of unknown function precursor, putative adhesin | 15.50 | 10.33 | 0.67 | ||
| THC0290_0743 | gldN | Gliding motility protein precursor GldN | 13.29 | 62.00 | 4.67 | |
| THC0290_1047 | Protein of unknown function precursor, putative adhesin | TIGR04131 | 13.00 | 4.33 | 0.33 | |
| THC0290_0500 | Probable S8 and S53 subtilisin family serine endopeptidase precursor | 10.53 | 52.67 | 5.00 | ||
| THC0290_1615 | Protein of unknown function | 10.29 | 24.00 | 2.33 | ||
| THC0290_1494 | Probable ribonuclease | TIGR04183 | 10.25 | 13.67 | 1.33 | |
| THC0290_0129 | Putative outer membrane protein precursor | TIGR04183 | 8.50 | 22.67 | 2.67 | |
| THC0290_0175 | Probable cell surface protein (Leucine-rich repeat protein) | TIGR04183 | 7.75 | 10.33 | 1.33 | |
| THC0290_0238 | fpp2 | Psychrophilic metalloprotease Fpp2 precursor | TIGR04183 | 6.15 | 67.67 | 11.00 |
| THC0290_2158 | Uncharacterized protein precursor. Probable phage protein. | 6.00 | 18.00 | 3.00 | ||
| THC0290_0754 | Protein of unknown function precursor, putative adhesin | 5.62 | 24.33 | 4.33 | ||
| THC0290_1616 | Protein of unknown function precursor, putative adhesin | TIGR04131 | 5.56 | 16.67 | 3.00 | |
| THC0290_0300 | Probable M36 fungalysin family metalloprotease precursor | TIGR04183 | 5.40 | 9.00 | 1.67 | |
Proteins which relative abundance changed at least ±2-fold significantly (p < 0.01) and for which at least 3 spectra have been identified in one of analyzed strain (full data available in Table S1).
Proteins annotation as previously described (Rochat et al., 2017a).
CTD type identified by BLASTP analysis.
Protein abundance ratio calculated using spectral counts.
Average number of spectral counts calculated using triplicates values for each strain.