Table 5.

Surfome of strains THCO2-90 and gldG::Tn identified by LC-MS/MS analysis^a.

Locus tag	Gene	Predicted functionb	CTDc	Ratio TH/gldGd	Spectrum count averagese
					TH	gldG::Tn
THC0290_2147	gldJ	Gliding motility lipoprotein precursor GldJ		Absent	27.00	0
THC0290_2385		RCC1 (Regulator of Chromosome Condensation) repeat domain protein precursor	TIGR04183	Absent	5.67	0
THC0290_0091		Probable glycoside hydrolase precursor	TIGR04183	Absent	4.33	0
THC0290_0232	hppD	4-hydroxyphenylpyruvate dioxygenase		Absent	3.67	0
THC0290_1494		Probable ribonuclease	TIGR04183	Absent	3.33	0
THC0290_1252		Universal stress protein, UspA family		Absent	3.00	0
THC0290_1520		Probable endonuclease precursor	TIGR04183	Absent	3.00	0
THC0290_2305	fabH3	3-oxoacyl-[acyl-carrier-protein] synthase III protein FabH3		Absent	3.00	0
THC0290_0740	gldK	Gliding motility lipoprotein precursor GldK		16.0	10.7	0.7
THC0290_0129		Putative outer membrane protein precursor	TIGR04183	11.3	15.0	1.3
THC0290_0743	gldN	Gliding motility protein precursor GldN		6.8	11.3	1.7
THC0290_0931		Collagenase precursor		2.7	33.7	12.3
THC0290_2146	porU	Por secretion system protein PorU precursor. C-terminal signal peptidase		0	0.00	18.33
THC0290_1795		Probable lipoprotein precursor		0	0.00	17.67
THC0290_0025	sprB	Putative adhesin precursor SprB (modular protein)	TIGR04131	0	0.00	8.33
THC0290_0946	pafA	Alkaline phosphatase precursor		0	0.00	8.00
THC0290_0866		Protein of unknown function		0	0.00	5.67

^aProteins which relative abundance changed at least ±2-fold significantly (p < 0.01) and for which at least 3 spectra have been identified in one of analyzed strain (full data available in Table S2).

^bProteins annotation as previously described (Rochat et al., 2017a).

^cCTD type identified by BLASTP analysis.

^dProtein abundance ratio calculated using spectral counts.

^eAverage number of spectral counts calculated using triplicates values for each strain.