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. 2017 Nov 7;8:802. doi: 10.3389/fphar.2017.00802

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Copyright © 2017 García-Caballero, Martínez-Poveda, Medina and Quesada.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AD0157 induces chromatin condensation and DNA fragmentation in HL60, U937 and KU812F cells. (A) Effect of AD0157 on human myeloid cell morphology and chromatin condensation evaluated by Hoechst staining (bar = 50 μm). Graphs represent the percentage of control and AD0157-treated cells showing condensed chromatin. Values are expressed as means ± SD of the counts evaluated in ten vision fields. ^∗∗p < 0.001 versus untreated cells. (B) Effect of AD0157 on DNA fragmentation assessed by TUNEL labeling (bar = 50 μm). Graphs represent the percentage of control and AD0157-treated showing TUNEL positivity. Values are expressed as means ± SD of the counts evaluated in ten vision fields. ^∗∗p < 0.001 versus untreated cells.