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. 2017 Nov 7;8:802. doi: 10.3389/fphar.2017.00802

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Copyright © 2017 García-Caballero, Martínez-Poveda, Medina and Quesada.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AD0157 induces the release of cytochrome c into the cytosol and suppresses Bad phosphorylation. (A) Detection of cytochrome c in the cytosolic (supernatant) and mitochondrial (pellet) fractions of AD0157-treated and non-treated leukemia cells. HL60, U937 and KU812F cells incubated with AD0157 were suspended in MSH buffer containing EGTA, succinate and MgCl_2, permeabilized with digitonin and the cytosolic and mitochondrial fractions were separated and subjected to Western blots. (B) Percentage of cytochrome c in the pellet (mitochondria) and supernatant (cytosol) after treatment with AD0157, expressed as means ± SD of three independent assays. ^∗∗p < 0.001 versus control. (C) Bad phosphorylation status in treated and untreated HL60, U937 and KU812 cells. Representative blots of p-Bad and total Bad in the studied leukemia cell lines. (D) Percentage of p-Bad/Bad ratios expressed as means ± SD of three independent assays. ^∗∗p < 0.001 versus control.