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. 2017 Nov 7;8:905. doi: 10.3389/fphys.2017.00905

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Copyright © 2017 Fu, Zhang, Xiao, Basheer, Baum, Epifantseva, Hong and Shaw.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GJA1-20k delineates the mitochondrial network. (A) Imaging of live HeLa cells expressing GFP-tagged GJA1-43k or GJA1-20k (green) and mitochondrial markers (red). GJA1-43k associates with mitochondria as small puncta (arrows). GJA1-20k colocalizes with mito-BFP (middle panel) and TMRM (bottom panel). Scale bar = 5 μm. (B) Relative enrichment of HA-tagged isoforms in total cell lysate (input), the cytosol supernatant (F0), and membrane fractions of crude mitochondrial pellet (F1–F3). Mitochondria (Tom20). Plasma membrane (Na⁺K⁺ATPase, N-cadherin). Full blots were in Figures S7, S8. (C) Live cell imaging of HaCat and HEK293T cells cotransfected with mito-BFP and GJA1-20k-GFP. Immunofluorescence of GFP and Tom20 in primary glial cells and cardiac fibroblasts transfected with GJA1-20k-GFP. Scale bar = 5 μm.