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. 2017 Nov 7;8:905. doi: 10.3389/fphys.2017.00905

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Copyright © 2017 Fu, Zhang, Xiao, Basheer, Baum, Epifantseva, Hong and Shaw.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GJA1-20k binds α-tubulin, targeting to the microtubule/mitochondria interface. (A) Coimmunoprecipitation between GJA1-20k-HA (IP) and α-tubulin (IB). Antibodies used for IP were labeled as mGST (mouse GST antibody) and m20kHA (mouse HA antibody). Full blot is in Figure S9. (B) Live-cell confocal of GJA1-20k-mCherry and α-tubulin-GFP in HeLa cells. Individual GJA1-20k tracks (45 s). (C) Time-lapse of merged and tubulin images with moving mitochondria. (D) TEM images of GJA1-20k-GFP (immunogold labeled for GFP) occurring along a microtubule bundle, a mitochondrion, and the interface between these structures (arrows) in HeLa cells. Non-transfected negative control (left panel). Microtubules (mt). Mitochondria (M). Scale bar = 500 nm. (E) The percent of total images (145) with positive immunogold labeling of each respective organelle is listed in the table. A total of 145 images with gold particles are analyzed.