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. 2017 Oct 29;2017:6109061. doi: 10.1155/2017/6109061

Figure 1.

Figure 1

IEX-1-mediated IPC-induced cardioprotection. (a) IEX-1 mRNA level in rat hearts post-IPC was detected by quantitative real-time PCR (n = 5~6). (b) IEX-1 mRNA level in rat hearts postischemia (30 min) or I/R (30 min/30 min). (c) IEX-1 mRNA level in rat hearts subjected to sham operation (Sham), I/R (30 min/3 h), or IPC prior I/R (n = 6~10). (d). 5 μg scrambled siRNA (csiR) or siRNA targeting IEX-1 (siIEX-1) with transfection reagent in 250 μl of saline was directly injected intramyocardially into the left ventricular muscular wall. 2 days after the injection, rats underwent I/R (30 min/24 h) or IPC prior I/R (n = 6~7), then hearts were excised and sliced for infarction analysis by Evans blue and triphenyltetrazolium chloride (TTC) attaining; (e) percentage of the area at risk (AAR) to the left ventricle (LV) and (f) infarct area (MI) to AAR were determined by computer-assisted planimetry. (g) Serum lactate dehydrogenase (LDH) and (h) creatine kinase (CK) activity were measured at the end of 24-hour reperfusion. n = 7~10, ∗P < 0.05 versus csiR + I/R, #P < 0.05 versus csiR + IPC + I/R.