Figure 6.

IEX-1 overexpression inhibits H/R-induced ROS accumulation. Neonatal rat cardiomyocytes were infected with adenovirus at 10 MOI for 36 h, then underwent H/R (4 h/30 min). (a) ROS levels measured by luminol chemiluminescence (left). The column data are the sum counts of the 30-minute measurement period (right). (b) Intracellular ROS were detected by DCFH-DA (scale bar = 40 μm), and (c) mitochondrial ROS were detected by MitoTracker Red CM-H2XRos probe with confocal microscopy (scale bar = 20 μm). ^∗P < 0.05 versus Ctrl, ^#P < 0.05 versus GFP or β-Gal.