Figure 3. Species Accumulation Curves (SACs) across survey nights showing differences between single strategic and spatially replicated acoustic survey methods: (A–B) overall (across both pinelands and prairies), (C–D) in pinelands and (E–F) in prairies.

Gray shading represents 84% confidence intervals (Gotelli & Colwell, 2011). Gray cross-bars indicate the point at which an asymptote was reached for each SAC, and the corresponding estimated species richness (y-axis) and sample effort (x-axis). All data were collected in 2015 in Everglades National Park, FL, USA.