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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Mol Cancer Res. 2017 Jul 5;15(10):1410–1420. doi: 10.1158/1541-7786.MCR-17-0016

Figure 2. In vitro viral infection, cytopathic effects and replication of MV-uPA on human and murine fibroblast.

Figure 2

(A) Upper panel: uPAR expression in human primary cancer associated fibroblast CAF19, CAF23 and NIH-3T3 cells (immunocytochemistry). Scale bar = 20 μm. Mid and lower panels: CAF19, CAF23 and 3T3 species specific MV-uPA infection and virus induced GFP expression. Cells were photographed 48 h after infection (MOI=1). Scale bar = 100 μm. (B), In vitro viral replication (24, 48, 72 hours) in human CAF19, CAF23 and mouse 3T3 fibroblasts. Results are displayed as the average (+/− SD) of triplicate experiments. Lower limit of detection was 8 TCID50/ml. (C), Time dependent, MV-h-uPA induced cytotoxicity in CAF19, CAF23, and MV-m-uPA induced cytotoxicity in murine 3T3 cells. Virus dose: MOI=1. Results are presented as percentage of controls. Bars represent averages +/− SD of triplicate experiments.