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. Author manuscript; available in PMC: 2017 Nov 12.

Published in final edited form as: Biomaterials. 2013 Nov 20;35(5):1496–1506. doi: 10.1016/j.biomaterials.2013.11.013

Morphology of keratinocytes on PCL/collagen nanofibrous matrices alone or with collagen gel coating. A–H) Immunofluorescent staining of intracellular cytoskeleton protein of F-actin (Red) and nuclei with DAPI (purple/blue) (A, C: PCL/collagen nanofiber alone; B, D: with collagen gel coating), and focal adhesion proteins of vinculin (Green) (E, G: PCL/collagen nanofiber alone; F, H: with collagen gel coating). Arrowhead: keratinocyte lamellipodium; arrow: spatial distribution of vinculin. I) Percentage of polarized keratinocytes (n=3) ** Statistically significant, p < 0.001

Morphology of keratinocytes on PCL/collagen nanofibrous matrices alone or with collagen gel coating. A–H) Immunofluorescent staining of intracellular cytoskeleton protein of F-actin (Red) and nuclei with DAPI (purple/blue) (A, C: PCL/collagen nanofiber alone; B, D: with collagen gel coating), and focal adhesion proteins of vinculin (Green) (E, G: PCL/collagen nanofiber alone; F, H: with collagen gel coating). Arrowhead: keratinocyte lamellipodium; arrow: spatial distribution of vinculin. I) Percentage of polarized keratinocytes (n=3) ** Statistically significant, p < 0.001