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. 2017 Oct 17;40(10):761–772. doi: 10.14348/molcells.2017.0104

Fig. 4. Effect of SLC8A2 on the mRNA transcription and protein expression related to invasion and migration as well as the enzyme activity of MMP-2 in U87MG cells.

Fig. 4

One representative result of three independent preparations is shown. (A) RT-PCR was conducted to detect the mRNA expression of MMP-2, MMP-9, uPA and its receptor uPAR in U87MG, U87MG-NC and U87MG-SLC8A2 cells. GAPDH served as the internal control. (B) The protein levels of MMP-2 in U87MG, U87MG-NC and U87MG-SLC8A2 cells were examined by Western blot. GAPDH served as the loading control. (C) The activity of MMP-2 secreted in the DMEM was determined by gelatin zymography. (D) The protein levels of Snail, E-cardherin and vimentin in U87MG, U87MG-NC and U87MG-SLC8A2 cells were examined by Western blot. GAPDH served as the loading control. **P < 0.01. There was no significant difference between the U87MG and the U87MG-NC groups.