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. 2017 Nov 13;8:1439. doi: 10.1038/s41467-017-01636-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Ist1 controls Vps32 assembly on endosomes. a–d One-cell stage embryos expressing GFP::Cav1 and depleted of either Vps2 (n = 13, each condition) or Ist1 (n = 11, each condition) were fixed and stained using antibodies directed against GFP and either Hrs or Vps32 and imaged using confocal microscopy. Representative images are shown. Bars, 1 μm. e Quantification of the relative fluorescence intensities of Vps32 and Cav1 under various conditions based on STED imaging (see panel f). Standard error of the mean is reported in each case. ***p < 0.001 as compared to control, using a t-test; *p < 0.05 as compared to control, using a one-way ANOVA with Dunnett’s test. f One-cell stage control (n = 10, each condition) and ist1 (tm7401) mutant embryos (n = 20, each condition) expressing GFP::Cav1 in the presence or absence of Vps2 were fixed and stained using antibodies directed against GFP and Vps32 and imaged using STED microscopy. Arrows highlight the presence of subdomains containing Vps32. Representative images are shown. Bars, 5 μm (left panels) and 1 μm (right, zoomed panels)