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. 2017 Nov 13;8:1456. doi: 10.1038/s41467-017-01744-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Ectopic expression of CSDE1 reduces FABP7 levels and impairs neural differentiation. a qPCR analysis of FABP7 and VIM levels (relative expression to EV H9 hESCs) in CSDE1-overexpressing (CSDE1 OE) H9 hESCs. Data represent two independent experiments with three biological replicates. b Western blot analysis of H9 hESCs with antibodies to CSDE1, FABP7 and VIM. β-actin is the loading control. c After 10 days of neural induction, cells were assessed by immunofluorescence with OCT4, PAX6, and Hoechst staining. Scale bar represents 20 μm. d Quantification of the percentage of PAX6-positive cells/total nuclei after 10 days of neural induction. Graph represents the mean ± s.e.m. of three independent experiments, 1000–1400 total cells per experiment. e Western blot analysis of H9 hESCs and their differentiated counterparts after 10 days of neural induction. f qPCR analysis after 10 days of neural induction. Data (relative expression to EV shRNA cells) represent three independent experiments with three biological replicates. All the statistical comparisons were made by Student’s t-test for unpaired samples. P-value: *(P < 0.05), **(P < 0.01), ***(P < 0.001), ****(P < 0.0001)