Figure 10.

Emodin inhibits T cell proliferation and mTOR signaling in vitro. An MLR was set up using irradiated Balb/C splenocytes as stimulators and B6-derived T cells as responders in the absence or presence of CsA and/or or emodin. Either emodin or CsA inhibited T cell proliferation 3 (A) or 5 days (B) after MLR culture. Data are shown as means ± SD (n = 6 samples). One representative of three separate experiments is shown. Moreover, western blots for phosphorylated p70S6K and total p70S6K were performed two days after the similar MLR culture (C). GAPDH was used as a loading control. One representing image from three separate experiments is shown. OD values in column graphs were pooled from four separate experiments. Data are presented as means ± SD (n = 4 bands) after corrections by GAPDH. Results demonstrated that emodin mostly blocked the phosphorylation of p70S6K.