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. 2017 Nov 8;8:1519. doi: 10.3389/fimmu.2017.01519

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Copyright © 2017 Qiu, Liu, Liang, Nie and Dai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Emodin increases the frequencies and numbers of CD4⁺Foxp3⁺ regulatory T cells (Tregs). Draining lymph node (LN) and spleen cells from emodin- or CsA-treated B6 mice transplanted with BALB/c skin were isolated 10 days after transplantation. The frequency and absolute numbers of CD4⁺Foxp3⁺ Tregs from LNs and spleens of recipient mice treated with CsA and/or emodin were determined via a flow cytometer. Data of column graphs are shown as means ± SD (n = 6–8 mice). One of three separate experiments is shown.