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. 2017 Nov 8;8:1519. doi: 10.3389/fimmu.2017.01519

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Copyright © 2017 Qiu, Liu, Liang, Nie and Dai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Emodin also augments the percentages of CD8⁺CD122⁺ Tregs. Draining LN and spleen cells from emodin- or CsA-treated B6 mice transplanted with BALB/c skin were isolated 10 days after transplantation. The percentages of CD8⁺CD122⁺ Tregs from LNs and spleens of recipient mice were measured via a flow cytometer. Data are shown as means ± SD from two separate experiments (*P < 0.05 and **P < 0.01, n = 4–5 mice/group).