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. 2017 Nov 8;8:1519. doi: 10.3389/fimmu.2017.01519

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Copyright © 2017 Qiu, Liu, Liang, Nie and Dai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Depleting regulatory T cells (Tregs) largely reverses allograft survival extended by emodin. C57BL/6 mice received a skin graft from a BALB/c mouse and were treated with emodin or CsA. Tregs in recipient mice were depleted using anti-CD25 (PC61) and anti-CD122 (TMβ1). Skin allograft rejection was then observed (n = 6–7 grafts).