Oryza sativa |
pRGEB32 |
PTG1/Cas9 |
U3 snoRNA |
Rice ubiquitin |
Hygromycin |
Cut |
Enhanced multiplex editing capability via endogenous tRNA processing system |
Xie et al., 2015 |
Zea mays |
pHSE401 |
gRNA scaffold |
AtU6-26 |
35S |
Hygromycin |
Cut |
Improved designing of CRISPR/Cas9 binary vector. Easy method for one or more gRNA assembly in expression cassette, high efficiency mutant generation |
Xing et al., 2014 |
Arabidopsis thaliana |
pKI 1.1R |
Human codon optimized spCas9 |
U6-26p |
CaMV35S, WOX2, RPS5A |
Hygromycin |
Cut |
pKIR vector harboring RPS5A maintains high constitutive expression and heritable muatations at all developmental stages |
Tsutsui and Higashiyama, 2017 |
Arabidopsis, Nicotiana benthamiana, Oryza sativa |
pYPQ159 |
hSpCas9D10A (human codon optimized) |
AtU6-26 |
2× 35S |
Spectinomycin |
Nick |
This toolbox provides reagents to efficiently assemble DNA constructs for monocots and dicots using Golden Gate cloning |
Lowder et al., 2015 |
Zea mays, Arabidopsis |
pBUN501 |
zCas9D10A |
AtU6-26 |
Ubi1 |
Bar |
Nick |
Multiplex genome editing |
Xing et al., 2014 |
Zea mays, Arabidopsis |
pHSN6A01 |
dCas9-VP64, gRNA scaffold |
AtU6-26p |
2× 35Sp |
Hygromycin |
Activate |
Multiplex genome editing |
Xing et al., 2014 |
Zea mays, Arabidopsis |
pBUN6A11 |
dCas9-VP64, gRNA scaffold |
OsU3p |
Ubi1p |
Bar |
Activate |
Multiplex genome editing |
Xing et al., 2014 |
Arabidopsis, Nicotiana benthamiana |
pEGB 35S:dCas9:Tnos (GB1191) |
dCas9 |
AtU6-26 |
35S, Tnos |
Kanamycin |
Interfere |
GoldenBraid 2.0 validation- foster DNA exchange for synthetic biology; protein-protein interaction tool, gene silencing tool |
Perdigones et al., 2013 |
Nicotiana benthamiana |
pdCas9 (GB1079) |
Mutated Cas9 (D10A, H840A) and inactivated catalytic domains (human codon optimized) |
U6-26 |
pNOS |
Ampicillin |
Interfere |
GoldenBraid gRNA-Cas9 toolbox validated; multiple TDNA cloning, gRNA multiplexing; effective transcriptional activation and repression |
Vazquez-Vilar et al., 2016 |