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. 2017 Nov 8;8:811. doi: 10.3389/fphar.2017.00811

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Copyright © 2017 Vieira, Ferreirinha, Magalhães-Cardoso, Silva, Marques and Correia-de-Sá.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD11B-immunoreactive inflammatory cells surrounding myenteric ganglia and infiltrating the intramuscular layer of TNBS-treated rats also stain positively against CD206 (a cell marker of anti-inflammatory M2 macrophages) and tetramethylrhodamine-conjugated α-bungarotoxin (BTX-rhod) identifying α7 subunit-containing nicotinic receptors both in Control (a,c,e,g) and TNBS-treated (b,d,f,h) rats. Confocal micrographs of whole-mount preparations of the longitudinal muscle-myenteric plexus of the ileum of Control (i) and TNBS-treated (j) rats show that CD11B-positive cells do not express the P2X7 receptor. Images are representative of at least four animals per group. Scale bar = 100 μm.