Figure 2.

Exendin-4 effects on apoptosis, cell viability and signaling pathway. Ovarian cancer cell lines were stimulated for 24 h with Exendin-4 (50 nM), Exendin-4 (50 nM) pre-treatment for 24 h and Camptothecin (CPT, 100 nM) in both SKOV-3 (A, B, C) and CAOV-3 (D, E, F). Caspase 3/7 activation in SKOV-3 (A) and CAOV-3 (D), cell viability in SKOV-3 (B) and CAOV-3 (E) and phosphoIκB in SKOV-3 (C) and CAOV-3 (F) was analyzed. Relative fluorescence units (RFU). Mean values ± s.e.m. are shown. n = 3 per group. *P < 0.05, **P < 0.01, ***P < 0.001 Control vs different conditions. One-way ANOVA followed with Dunnett’s post hoc.