Figure 11.

Effects of Cd and/or Tre on lysosomal proteolytic activity in rPT cells. (a) Cells grown on coverslips were preincubated with 10 μg/ml DQ-BSA green for 12 h, and then refreshed the medium and treated with 2.5 μM Cd and/or 5 mM Tre for 12 h to evaluate the lysosomal degradation capacity using confocal microscopy. (b) Cells were treated with 2.5 μM Cd and/or 5 mM Tre for 12 h, and then protein levels of CTSB and CTSD were assessed by western blot analysis. Upper panel representative western blot image; lower panel quantitative analysis of protein levels (mean±S.E.M., n=4). *P<0.05; **P<0.01