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. 2017 Oct 12;8(10):e3099. doi: 10.1038/cddis.2017.475

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effect of Tre on Cd-induced apoptosis in rPT cells. (a and b) Cells grown on coverslips were coincubated with 2.5 μM Cd and 5 mM Tre for 12 h, and nuclear chromatin changes (apoptosis) were assessed by DAPI staining. Changes of nuclei fragmentation with condensed chromatin are evident (thin arrows). Representative morphological changes of apoptosis are present in (a), and its statistical result of apoptotic rates (b) are expressed as mean±S.E.M. (n=9). (c and d) Cells were treated with Cd and/or Tre for 12 h to assess the apoptosis using flow cytometry. Representative dot plots of Annexin V-PI staining are present in (c), and its statistical result of apoptotic rates (d) are expressed as mean±S.E.M. (n=9). NS, not significant; **P<0.01