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. 2017 Oct 12;8(10):e3099. doi: 10.1038/cddis.2017.475

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Effects of Cd and/or Tre on autophagic flux in rPT cells. Cells grown on coverslips were transfected with RFP-GFP-LC3 plasmid for 36 h, and then treated with 2.5 μM Cd and/or 5 mM Tre for 12 h. (a) Representative confocal images of different treatments as indicated. (b) The number of yellow puncta (autophagosomes) and the number of red puncta (autolysosomes) in the merged images were counted and the total number of puncta per cell was calculated as percentage. Data are presented as mean±S.E.M., n=3 independent experiments; *P<0.05; **P<0.01