Figure 4.

The influence of miR-124 or iASPP on p53^wt/p53^mut and p53^+/+/p53^−/− cells in response to PDT treatment. (a) and (b) miR-124 mimics or miR-124 inhibitor was transfected into RKO (p53^wt), HT29 (p53^mut), p53^+/+ or p53^−/− cells to achieve miR-124 overexpression or miR-124 inhibition. The transfection efficiency was verified by using real-time PCR assays. (c) The viability of p53^wt CRC cell line RKO and p53^mut cell line HT 29 transfected with miR-SCR or miR-124 in the absence or presence of PDT was determined by using CCK-8 assays. (d) The viability of p53^+/+ CRC cell line and p53^−/− cell line transfected with miR-SCR or miR-124 in the absence or presence of PDT was determined by using CCK-8 assays. (e) The viability of p53^wt CRC cell line RKO and p53^mut cell line HT 29 transfected with pcDNA3.1 or pcDNA3.1-iASPP in the absence or presence of PDT was determined by using CCK-8 assays. (f) The viability of p53^+/+ CRC cell line and p53^−/− cell line transfected with pcDNA3.1 or pcDNA3.1-iASPP in the absence or presence of PDT was determined by using CCK-8 assays. The data are presented as mean±S.D. of three independent experiments. *P<0.05, **P<0.01, miR-124 mimics versus mimics-NC, or iASPP versus pcDNA3.1; #P<0.05, ##P<0.01, HT29 versus RKO, or p53^−/− HCT116 versus p53^+/+ HCT116; &P<0.05, &&P<0.01, PDT (+) group versus PDT (−) group