Figure 2.

Knockout of hsf4 in zebrafish caused early-onset cataract. (a) Slit lamp examination of the WT and hsf4^null zebrafish. Opacity of the eye lens could be detected from hsf4^null zebrafish at all the ages we examined. However, the WT lens were transparent. (b) DAPI staining of the WT and hsf4^null zebrafish lens at 2, 3, 5 and 7d. Scale bar: 20 μm. (c) Enlarged image of white box in the lens of 5-d-old hsf4^null lens in b. (d) Enlarged image of white box in the lens of 7-d-old hsf4^null lens in b. The white arrows in c and d indicate the spherical nucleus. Scale bar: 20 μm. (e) DAPI staining of the adult WT and hsf4^null zebrafish lens at 2 and 6M. The middle column is the enlarged images of white boxes in 2M WT and hsf4^null lens. The white arrows indicate the undegraded nucleus deposited in the hsf4^null lens. The red arrows indicate the spherical nucleus existing in hsf4^null lens. Scale bar: 500 μm