Figure 4.

Progressively disordered arrangement of lens fiber cells in the in the hsf4^null zebrafish lens. Immunostaining the WT and hsf4^null zebrafish lens from different ages with antiphalloidin antibody. (a) The signals are more denser in the hsf4^null zebrafish than the WT zebrafish at 2M of age. (b) The fiber cell structures becoming more disorganized in the hsf4^null zebrafish at 6M of age. (c) The irregular signal disappeared in the hsf4^null zebrafish at 8M of age. The white arrows indicate the mislocated fiber cell nucleus in the anterior region of the lens. Scale bar: 20 μm