Figure 2.

miR-223 inhibits myoblast proliferation. (a) CCK-8 assay indicates miR-223 overexpression inhibited chicken primary myoblast (CPM) proliferation. (b) CCK-8 assay indicates miR-223 inhibition promoted CPM proliferation. (c) CPM was overexpressed with miR-223 and the negative control (NC) mimic, and the cell cycle phase was then analyzed. (d) Relative mRNA expression of the cell cycle-related genes after transfection of miR-223 and NC mimic. (e) CPM was transfected with miR-223 inhibitor and the NC inhibitor, and the cell cycle phase was then analyzed. (f) Relative mRNA expression of the cell cycle-related genes after transfection of miR-223 inhibitor and NC inhibitor. (g) EdU staining after transfection of miR-223 mimic and miR-223 inhibitor. Bar, 50 μm. (h) The proliferation rate of myoblasts transfected with miR-223 mimic and miR-223 inhibitor. (i) CCK-8 assay showed that miR-223 overexpression inhibited qm-7 proliferation. (j) CCK-8 assay showed that miR-223 inhibition promoted qm-7 proliferation. (k) qm-7 was overexpressed with miR-223 and the NC mimic, and the cell cycle phase was then analyzed. (l) Relative mRNA expression of the cell cycle-related genes after transfection of miR-223 and NC mimic. (m) qm-7 was transfected with miR-223 inhibitor and the NC inhibitor, and the cell cycle phase was then analyzed. (n) Relative mRNA expression of the cell cycle-related genes after transfection of miR-223 inhibitor and NC inhibitor. (o) EdU staining after transfection of miR-223 mimic and miR-223 inhibitor. Bar, 50 μm. (p) The proliferation rate of qm-7 cells transfected with miR-223 mimic and miR-223 inhibitor. Results are shown as the mean±S.E.M. of three independent experiments. Independent sample t-test was used to analysis the statistical differences between groups. *P<0.05; **P<0.01