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. 2017 Oct 5;8(10):e3094. doi: 10.1038/cddis.2017.479

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Copyright © 2017 The Author(s)

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The inhibitory effect of miR-223 on myoblast proliferation was achieved by its target gene IGF2. (a) The potential binding site of miR-223 in the IGF2 mRNA 3′ UTR. (b) Dual-luciferase reporter assay indicated that miR-223 can bind to the predicted binding site of the IGF2 mRNA 3′ UTR. (c) miR-223 overexpression significantly inhibited IGF2 mRNA and protein expression in CPM. (d) miR-223 inhibition significantly promoted IGF2 mRNA and protein expression in CPM. (e) IGF2 overexpression significantly promoted IGF2 mRNA and protein expression in CPM. (f) IGF2 inhibition significantly inhibited IGF2 mRNA and protein expression in CPM. (g) IGF2 overexpression significantly promoted IGF2 mRNA and protein expression level in qm-7. (h) IGF2 inhibition significantly inhibited IGF2 mRNA and protein expression level in qm-7. (i) CCK-8 assay indicates that IGF2 overexpression significantly promoted myoblast proliferation in CPM. (j) CCK-8 assay indicates that IGF2 overexpression significantly promoted myoblast proliferation in qm-7. (k) CPM was overexpressed with IGF2 and the control vector, and the cell cycle phase was then analyzed. (l) qm-7 was overexpressed with IGF2 and the control vector, and the cell cycle phase was then analyzed. (m) CCK-8 assay indicates that IGF2 inhibition significantly reduced myoblast proliferation in CPM. (n) CCK-8 assay indicates that IGF2 inhibition significantly reduced myoblast proliferation in qm-7. (o) CPM was transfected with IGF2 siRNA and the NC siRNA, and the cell cycle phase was then analyzed. (p) qm-7 was transfected with IGF2 siRNA and the NC siRNA, and the cell cycle phase was then analyzed. (q). CPM was co-transfected with miR-223 and pSDS-IGF2, miRNA mimic NC and control vector, miR-223 and control vector, respectively, and the cell cycle phase was then analyzed. Results are shown as the mean±S.E.M. of three independent experiments. Independent sample t-test was used to analysis the statistical differences between groups. *P<0.05; **P<0.01