Figure 6.

The inhibition of miR-223 on IGF2 and ZEB1 is different between myoblast proliferation and differentiation. (a) Relative IGF2 expression level during CPM differentiation. (b) Relative IGF2 expression level during qm-7 differentiation. (c) Expression level of muscle differentiation marker genes after CPM transfected with IGF2 siRNA. (d) Expression level of the muscle differentiation marker genes after qm-7 transfected with IGF2 siRNA. (e) CPM was transfected with ZEB1 siRNA and the NC siRNA, and the cell cycle phase was then analyzed. (f) qm-7 was transfected with ZEB1 siRNA and the NC siRNA, and the cell cycle phase was then analyzed. (g) The proliferating (GM) and differentiating (DM) CPM were transfected with miR-223 mimic, respectively, and the relative ZEB1 and IGF2 mRNA expression levels were then analyzed. (h) Relative miR-223 expression in proliferating and differentiating CPM after transfected with miR-223 and NC mimic. (i) The proliferating and differentiating CPM were transfected with pmirGLO-ZEB1-3′UTR, pmirGLO-IGF2-3′UTR or pmirGLO, and the relative luciferase activity was then analyzed. Results are shown as the mean±S.E.M. of three independent experiments. Independent sample t-test was used to analysis the statistical differences between groups. ns, no significant. *P<0.05; **P<0.01