Figure 3.

miR-143 and miR-145 inhibit MYO6 expression by targeting its 3′-UTR. (a) mRNA expression levels of MYO6 (predicted target gene) in three gastric cancer cell lines, normal gastric epithelial cells and low and high-metastatic gastric cancer cell lines. (b) The protein expression level of MYO6 in SGC7901 and BGC823 cells after transfection with miR-143 and/or miR-145 mimics or miR-ctrl. (c) Diagram of the MYO6 3′-UTR-containing reporter construct. Mutations were generated at three predicted miR-143 and miR-145 binding sites located in the MYO6 3′-UTR. (d) Representative luciferase activity in BGC823 and AGS cells co-transfected with wild-type or mutated reporter plasmids and miR-ctrl, miR-143 or miR-145. *P<0.05, **P<0.01, ***P<0.001