Figure 1.

Screen for acridine derivatives that inhibit autophagic degradation. (a) Structures of a series of small molecules with an acridine skeleton and structure similar to chloroquine. (b) DLD1 cells were treated with 5 μM acridine derivative or 10 μM chloroquine for 24 h. Immunofluorescence was performed after staining with an anti-MAP1LC3 antibody. Scale bars, 5 μm. (c) Quantification of the MAP1LC3-positive punctate cells shown in (b). Only cells with more than five puncta were counted. Data represent the means±S.D. (n=3)